• Created by Unknown User (lylaatta@mit.edu), last modified by Unknown User (amgarcia@mit.edu) on Aug 15, 2014 09:28

pENTR LilrB2:

  • Blunt end cloning of gBlock
  • PCR screen of product

  • Miniprep sample with correct band pattern
  • Sequence verify
  • Proceed with LilrB2 constructs


pEXPR hEF1a:PirB-YFP

  • Transform
  • Grow up midi culture
  • Midiprep
  • Make cell stock

 


 

 

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