Adapted from: http://openwetware.org/wiki/Sauer:Electrocompetent_cells

This is for transforming E. coli

  1. Remove cells from -80 and chill on ice for 10 minutes or until the culture is thawed
    1. Meanwhile: label 1 1.5 microcentrifuge tube per transformation and place aside
  2. Add 1 ul of plasmid DNA or __ ul of ligation to the competent cells
  3. Flick to mix
  4. Transfer to a fresh electroporation cuvette using a pipette
  5. Tap the tube on the bench to make sure all the culture reaches the bottom of the cuvette
  6. Electroporate based on the machine's specs
    1. For E. coli in 1 mm cuvettes- use the EC1 program
  7. IMMEDIATELY ADD 1 ML OF SOC TO THE CUVETTE AND TRANSFER TO A 1.5 ml MICROCENTRIFUGE TUBE USING THE INCLUDED PASTEUR PIPETTE
    1. THIS IS ESSENTIAL. 3X DROP IN EFFICIENCY PER MINUTE WAITED TO ADD SOC
  8. Shake the 1.5 ml centrifuge tube at the appropriate temperature for 1 hr.
  9. Pellet the cells by centrifuging at 4000 rpm for 3 min
  10. Gently dump the supernatant except for 150 ul
  11. Resuspend the pellet in the remaining 150 ul
  12. Plate on the appropriate plates
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