How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
miRNA Sensors (Lab Work)
- Mini prep
- pEXPR: MAV1212-TRE-L7ae
- Digest and run gel
- pEXPR: MAV1212-TRE-L7ae
- Inoculate midi (if digest is okay)
- pEXPR: MAV1212-TRE-L7ae
- Midi prep
- MAV1212-hEF1a-GAL4VP16
- MAV1212-TRE-GAL4VP16
- All the single input sensors
- Pick colonies
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Plasmids from BCR group
- Plasmids from protein receptor group
- Plasmids from Brian
- Analyze sequencing results
- All the sensors (round 2)
- Transform
- Low Sensor miR-144-181c
- Transfect
- TAL14 Experiment (Take 1)
- Seed
- Experiment #3 (Take 1)
- Experiment #4 (Take 2)
NOTES