BCR 2.0 Parts Library

Created by Unknown User (clrichar@mit.edu), last modified on Jul 07, 2015 14:36

Golden Gate Parts

TEVp PCR
tTEVp PCR
Syk PCR
Q3-RFP-Q4
Q8-12-Q6
Q8-3-Q6
Q8-9-Q6
Q8-6-Q6
3-TCS
6-TCS
9-TCS
12-TCS
15-TCS
TCS-3
TCS-6
TCS-9
TCS-12
TCS-15
GG DONR
GG DONR

LR Parts

Syk-eYFP
Gmab Heavy
pENTR 6 (Velia)
pENTR 10 (Velia)
Lyn-TEVp (iGEM)
Gmab-Light
Lyn
Syk-eYFP
Syk
mKate
Lyn-mKate
CD79A-TCS-Gal4VP16 (iGEM)
CD79A
hEF1a-LacO:
TRE:
Gal4VP16
hEF1a:
LacI
Syk-TEV (iGEM)
CMV5-CuO:
hEF1a-CuO:
CymR
pDEST 1-2
pDEST 2-3
pDEST 4-5
CD79B

pEXPRs

hEF1a:CD79B-pTet:RFP-Gal4VP16
UAS:eYFP
pEXPR 106 (Velia)
pEXPR 8 (Velia)
hEF1a:CD79A-TCS-Gal4VP16
TRE:Syk-TEV (iGEM)
hEF1a:LacI
hEF1a:mKate
TRE:CD79B
hEF1a:Gal4VP16
hEF1a:Gmab Light
TRE:CD79B
TRE:CD79A
TRE:Gmab Light
hEF1a:Gmab Heavy
hEF1a:eBFP
pLac:mKate (pLac = hEF1a-LacO)
TRE:Gal4VP16

Undiluted Midis

hEF1a:Gal4VP16
hEF1a:CD79B-TCS-Gal4VP16 (iGEM)
UAS:eYFP
hEF1a:mKate
TRE:Gal4VP16
hEF1a:rtTA
hEF1a-LacO:mKate (pLac: MKate is the same thing)
TRE:mKate
hEF1a:LacI
UAS:mKate
hEF1a:CD79A
hEF1a:CD79B
hEF1a:Lyn
hEF1a:GMab Heavy
hEF1a:GMab Light
hEF1a:CD79A-TCS-Gal4VP16 (iGEM)
TRE:Syk-TEVp (iGEM)

TC Working Midis

hEF1a:rtTA
hEF1a:mKate
hEF1a:eBFP
UAS:eYFP
TRE:Gal4VP16

Undiluted, Unannealed Linkers

Sequencing Primers

CD79A-TCS-Gal4VP16 S1
CD79A-TCS-Gal4VP16 S2
CD79A-TCS-Gal4VP16 S3
CD79A-TCS-Gal4VP16 S4
CD79B-TCS-Gal4VP16 S1
CD79B-TCS-Gal4VP16 S2
CD79B-TCS-Gal4VP16 S3
CD79B-TCS-Gal4VP16 S4
TRE:Syk-RFP-TEVp S1
TRE:Syk-RFP-TEVp S2
TRE:Syk-RFP-TEVp S3
TRE:Gal4VP16 S1
TRE:Gal4VP16 S2

PCR/SDM Primers

CD79B SDM F1
CD79B SDM R1
CD79B SDM F2
CD79B SDM R2
CD79B SDM F3
CD79B SDM R3
Gal4VP16 SDM F4/5 (Origionally labelled CD79B SDM F5, in some cases labelled 4 instead of 5 still same thing)
Gal4VP16 SDM R4/5 (Origionally labelled CD79B SDM R5, in some cases labelled 4 instead of 5 still same thing)
Syk F1
Syk R1
TEV F1
TEV R1
tTEV F1
tTEV R1
CD79B F3
CD79B R3

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