1. Thaw BP clonase on ice.
2. Mix 0.5 uL BP clonase with 1 uL appropriate pDONOR (typically ~150 ng/uL) and 1 uL attB-flanked PCR fragment (typically ~50 ng/uL).
3. Incubate at room temperature for 1-1.5 hours.
4. Ad 0.5 uL Proteinase K.
5. Incubate at 37 C 20 min.
6. Transform 1 uL into 10G competent cells.
7. Outgrow w/ SOC 1hr.
8. Plate on Kan, 30C overnight.