• Created by Unknown User (clrichar@mit.edu), last modified by Unknown User (lylaatta@mit.edu) on Aug 18, 2014 11:05
  1. aspirate off media
  2. add 500 uL PBS and aspirate
  3. Add 500 uL complete media
  4. add beta amyloid to 500 nM or other (500 nM=10)
  5. incubate in the incubator for 2hr
  6. aspirate media
  7. add 500 uL warm PBS and aspirate
  8. add 500 uL warm  complete media
  9. add streptavidin to a 10 ug/mL (5uL for our aliquot)
  10. Incubate in the dark for 1hr 30min
  11. aspirate media 
  12. add 500 uL of PBS
  13. Microscopy time! 
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