Antibodies
- Did a lot of PCR yesterday
- Manufacturer of Phusion mix used in PCR will detail what variables to use in PCR based on the primers and templates you are using
- Purify PCR product so that you can measure concentrations and use correct amounts for Golden Gate
Receptor
- Check that centrifuge is actually running
- Transfect today
- Cytometer on thursday
miRNA
- Meeting with Jake Beal
- depending on type of sensor you may be able to have 12 miRNA
- co-transfecting may be better than putting everything on the same plasmid
NEGEM NOTES
- Science is hard
- Our project is cool/really hard
- HP is really important
- ethics and legal stuff
- interview professionals, laymen, patients and families
- Potential delivery methods and what would treatment look like
- what is the current state of the art...
- Diagnosis?
- Treatment?
- BioBricks and the Parts Registry
- Mammalian Parts
- Overview
- What are our responsibilities?