Instructions

  1. Acquire 500 uL of freshly grown cells in liquid culture. This usually comes from the leftover culture that was grown for the miniprep. Fresh Culture can also be grown by inoculating a single colony in TB with appropriate antibiotic marker.
  2. Add 500 uL of culture to microtube.
  3. Add 500 uL of filter sterilized 50% v/v glycerol in water to culture.
  4. Label the tube!
  5. Place in appropriate box in -80C freezer.
  6. For anything important, make two cell stocks (by splitting or by doing the above twice) and store one in the backup cell stock box (Starting 6/28).
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