To Do:

  • Miniprep PY, LY, pENTR_TEVpCofilin
  • Verify Minipreps
  • Transform hEF1a:YFPCofilin
  • Pick TREt:YFPCofilin Colonies
  • LR pENTR_TEVpCofilin with hEf1a and TREt
  • LR LT 12 and PT 11 with hEf1a

 

All 4 pENTR_TEVpCofilin worked!

Gel

 

Of course, the others didn't work. Given the lack of blue colonies, I'm thinking backbone religation/only one part present (probably YFP). Need to figure out something else.

Sequencing:

LilrB2TCSG4 seems to have worked! 11 and 13 might have point mutations (high G content, unlikely). Will proceed with 12.
PirBTCSG4 seems to have worked, too! 13 seems to have a point mutation (high G content, unlikely). Will proceed with 11.

 

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