Transformed pEXPR hEF1a:LilrB2 and TREt:LilrB2 (10pm)
Digesting pENTR LilrB2YFP and LilrB2TCS
LilrB2YFP: PstI 3.1
LilrB2TCS: XhoI + XbaI CS
HL, LT (1-4), LY (1-4), LT(C)
HL, LT (1-4), LY (1-4), LY(C)
Looks like LT4 worked. Will send for sequencing (M13F and R)
The LYs don't seem to have worked. May be a problem with the YFP PCRs.
BTW, the gel was kinda sketch (chunky), that's why I loaded twice.
(why doesn't this rotate?!)