• Miniprep pEXPRs (TREt TC, hEF1a YC)
  • Check pEXPRs (TREt TC, hEF1a YC)
  • Inoculate Midipreps
  • Pick colonies for hEF1a LT, PT, TC

Digest TREtTC with ApaI Cutsmart
Digest hEF1aYC with BsrGI 2.1
Digest LT with BglI 3.1 <-- used the wrong enzyme, oops. Used BlpI, needs buffer CS


Digest TC with PvuII with 3.1
Digest LT11 with BlpI with CS

Give up on hEF1aYC. Won't be using it anyway.


pEXPR_TREt:TEVpCofilin is wrong. Gives weird patterns. Need to pick again.
pENTR:LilrB2TCSG4 is wrong. Big chunk is actually missing. Need to pick again.




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