Receptor Progress - July 8

Created by Unknown User (shinjini@mit.edu), last modified by Unknown User (lylaatta@mit.edu) on Jul 09, 2014 15:17

pEXPRs midiprepped
LR product digested and gel ran
PCRs for fusions in progress

Digesting LR's

Hef1a:LirB2 BsrGI

Hef1a:PirB ClaI

	Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)
Hef1a:LilrB2 1r	532.9	500	0.938262		Hef1a:LilrB2 1c	532.9	500	0.938262		Hef1a:PirB 1r	478.9	500	1.044059		Hef1a:PirB 1c	478.9	500	1.044059
H2O			16.06174		H2O			17.06174		H2O			15.95594		H2O			16.95594
Buffer 2.1			2		Buffer 2.1			2		CutSmart			2		CutSmart			2
BsrGI			1		BsrGI			0		ClaI			1		ClaI			0
Total			20		Total			20		Total			20		Total			20

	Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)
Hef1a:LilrB2 2r	166.5	500	3.003003		Hef1a:LilrB2 2c	166.5	500	3.003003		Hef1a:PirB 2r	526	500	0.95057		Hef1a:PirB 2c	526	500	0.95057
H2O			13.997		H2O			14.997		H2O			16.04943		H2O			17.04943
Buffer 2.1			2		Buffer 2.1			2		CutSmart			2		CutSmart			2
BsrGI			1		BsrGI			0		ClaI			1		ClaI			0
Total			20		Total			20		Total			20		Total			20

	Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)			Conc(ng/uL)	Mass (ng)	Volume(uL)
Hef1a:LilrB2 3r	242.6	500	2.061006		Hef1a:LilrB2 3c	242.6	500	2.061006		Hef1a:PirB 3r	536.1	500	0.932662		Hef1a:PirB 3c	536.1	500	0.932662
H2O			14.93899		H2O			15.93899		H2O			16.06734		H2O			17.06734
Buffer 2.1			2		Buffer 2.1			2		CutSmart			2		CutSmart			2
BsrGI			1		BsrGI			0		ClaI			1		ClaI			0
Total			20		Total			20		Total			20		Total			20

Gel

1 HyperLadder 1kb

2 Hef1a:LilrB2 1r

3 Hef1a:LilrB2 1c

4 Hef1a:LilrB2 2r

5 Hef1a:LilrB2 2c

6 Hef1a:LilrB2 3r

7 Hef1a:LilrB2 3c

8 Hef1a:PirB 1r

9 Hef1a:PirB1c

10 Hef1a:PirB 2r

11 Hef1a:PirB 2c

12 Hef1a:PirB 3r

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