• Plates from our transformation grew blue and white colonies
    • PirB2 didn't grow any white colonies 
  • Liquid culture - to make stock - of ggDONR from troubleshooting experiment 
  • We golden gated again
    • first, we have to PCR amplify our gblocks
  • Made liquid culture of GGDonr and pENTR_L1_LilrB2_L2 for miniprep
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