• Created by Unknown User (shinjini@mit.edu), last modified by Unknown User (lylaatta@mit.edu) on Jun 30, 2014 08:33
  • Digesting LilrB2, PirB and GGDonr (again). EcoRI this time
  •  Conc(ng/uL)Mass (ng)Volume(uL) Conc(ng/uL)Mass (ng)Volume(uL)
    pENTR_LilrB2 (old)80.95006.18047 pENTR_LilrB2 (new)52.45009.541985
    H2O  10.81953 H2O  7.458015
    CutSmart  2 CutSmart  2
    EcoRI  1 EcoRI  1
    Total  20 Total  20
             
     Conc(ng/uL)Mass (ng)Volume(uL) Conc(ng/uL)Mass (ng)Volume(uL)
    pENTR_PirB79.95006.257822 GGDONR96.95005.159959
    H2O  10.74218 H2O  11.84004
    CutSmart  2 CutSmart  2
    EcoRI  1 EcoRI  1
    Total  20 Total  20

  • 0 = Ladder

  • 1 = PirB
  • 2 = LilrB2 (new)
  • 3 = GGDONR
  • 4 = LilrB2 (old)

 

  • ggDONR plasmid has the correct band pattern - none of the others do 
    • could be recombination of Q sites
  • No labels