From UTexas protocol

Materials:

TE Buffer

Primer

 

You will first make a 100 uM master stock

1: spin down primer tube incase there is some primer stuck in cap

2: Calculate amount of buffer needed- multiply nmoles of primer by 10

3: add this many uL of TE to the primer tube

4: gently mix and allow to sit for 10 minutes before making a working stock

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