LR

pENTR L1_VP16Gal4_L2 obtained from lab library

LR 06/18

LR 06/22

Transformation

06/19

06/23

06/28

Pick Colonies

No colonies grew. Possibly because of a wrong TRE pENTR -> redo LR on 06/22

06/25

06/29

Miniprep, Digest, Sequence:

06/26 Gel doesn't seem promising - retransformed

06/30

Midiprep:

07/02

Gel:

06/26

06/30 - lane 3 


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