| Transfection Optimization | Dox/IPTG dose response curve | Linker optimization | Expression level optimization | Lyn or no Lyn |
|---|---|---|---|---|
June
| Monday 1st | Tuesday 2nd | Wednesday 3rd | Thursday 4th | Friday 5th | Saturday 6th | Sunday 7th |
| Monday 8th | Tuesday 9th | Wednesday 10th | Thursday 11th | Friday 12th | Saturday 13th | Sunday 14th |
| Monday 15th | Tuesday 16th | Wednesday 17th | Thursday 18th | Friday 19th | Saturday 20th | Sunday 21st |
| Monday 22nd | Tuesday 23rd | Wednesday 24th | Thursday 25th | Friday 26th | Saturday 27th | Sunday 28th |
| TRE:Gal4VP16 LR
| TRE:Gal4VP16 transformation | TRE:Gal4VP16 pick colonies | TRE:Gal4VP16 mini, digest, seq | analyze seq TRE:Gal4VP16 midi | Seed | |
| miniprep, digest, seq | analyze seq midi | |||||
CD79A miniprep, digest, seq
| CD79A analyze seq, midi CD79B-RFP-G4 SDM gel +transformation
| CD79B-RFP-G4 SDM pick colonies
| CD79B-RFP-G4 SDM miniprep, seq Syk-RFP-TEV PCR + gel | CD79B linker golden gates +transformation Syk-RFP-TEV golden gate +transform | CD79B linker golden gates pick colonies Syk-RFP-TEV pick colonies | |
pLac:Heavy pLac:Light LRs | pLac:Heavy pLac:Light transformations | pLac:Heavy pLac:Light pick colonies | pLac:Heavy pLac:Light mini, digest, seq | |||
| Monday 29th | Tuesday 30th | |||||
| transfect | + small molecule | |||||
CD79B linker golden gate mini, digest, seq Syk-RFP-TEV mini, digest, seq | ||||||
July
| Wednesday 1st | Thursday 2nd | Friday 3rd | Saturday 4th | Sunday 5th | ||
| cytometry | data analysis | TRANSFECTION OPTIMIZATION DATA DEADLINE | ||||
CD79B linker golden gate midi Syk-Linker-TEVp golden gates + transformation | Syk-Linker-TEVp pick colonies | Syk-Linker-TEVp mini, digest, seq | ||||
| Monday 6th | Tuesday 7th | Wednesday 8th | Thursday 9th | Friday 10th | Saturday 11th | Sunday 12th |
| transfect | + small molecule | cytometry | data analysis | SMALL MOLECULE DOSE RESPONSE DATA DEADLINE | ||
Syk-Linker-TEVp midi | ||||||
pLac:CD79A pLac:CD79B LR | pLac:CD79A pLac:CD79B transformations | pLac:CD79A pLac:CD79B pick colonies | ||||
| Monday 13th | Tuesday 14th | Wednesday 15th | Thursday 16th | Friday 17th | Saturday 18th | Sunday 19th |
| transfect | + small molecule | cytometry | data analysis | LINKER OPTIMIZATION DATA DEADLINE | ||
pLac:CD79A pLac:CD79B mini, digest, seq | pLac:CD79A pLac:CD79B pLac:Heavy pLac:Light midi | |||||
| Monday 20th | Tuesday 21st | Wednesday 22nd | Thursday 23rd | Friday 24th | Saturday 25th | Sunday 26th |
| transfect | +small molecule | cytometry | data analysis | EXPRESSION LEVEL OPTIMIZATION DATA DEADLINE | ||
| Monday 27th | Tuesday 28th | Wednesday 29th | Thursday 30th | Friday 30th | ||
| transfect | + small molecule | cytometry | data analysis | LYN OR NO LYN DATA DEADLINE |