06/16:

LR

06/18

transformation

06/19

pick colonies

06/22

miniprep

06/23

digest + gel

potential mix up in loading the gel, diges+gel repeated

06/25

digest + gel

reLR

06/29

digest + gel of LR1 with different enzymes

cultured and miniprep LR2

07/01

midi culture

07/02

midi

Gel

6/23

Notes:

Band pattern doesn't match either a successful LR or a failed one. Band pattern matches pLac:mKate which was run on the same gel, indicating that samples may be loaded in the wrong order.

 
Perfect.

 

Sequencing results

 

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