PCR of TCS-Gal4VP16

Gel shows successful PCR of TCS-Gal4VP16 from pEXPR hEF1a-FGFR-mKate-TCS(L)-G4.

Forward primer:  iGEM KRB 001 F

Reverse primer:  iGEM KRB 002 R

Annealing Temperature:  63C?

Run using samples after PCR purification.

PCR of CD79B-TCS (no DMSO)

Gel shows unsuccessful PCR of CD79B-TCS from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B Qlinker_R

Annealing temperature:  70C

Run using samples after PCR purification.

PCR of CD79B-TCS (5% DMSO)

Gel shows unsuccessful PCR of CD79B-TCS from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B Qlinker_R

Annealing temperature:  67C

Run using samples after PCR purification.

PCR of CD79B-TCS (with betaine)

Gel shows unsuccessful PCR of CD79B-TCS from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS001 CD79B Q1_F

Reverse primer:  iGEM_AS002 CD79B Qlinker_R

Annealing temperature:  ?

Run using samples after PCR purification.

PCR of CD79B-TCS

Gel shows successful PCR of CD79B-TCS from pENTR CD79B (pDONR223).

Forward primer:  iGEM_AS012 CD79B Q1_F

Reverse primer:  iGEM_AS014 CD79B Qlinker_R

Annealing temperature:  ?

Run using samples after PCR purification.  Extra bands for CD79B-TCS indicated multiple PCR products, used gel extraction protocol to remove the appropriate band.

Restriction Digest Verification of pENTR

7 July 2014:

See gel here