PCR of Syk-(15aa)

Gel shows unsuccessful PCR of Syk-(15aa) from pENTR Syk (pDONR223).

Forward primer:  iGEM_AS006 Syk Q1_F

Reverse primer:  iGEM_AS008 Syk Qlonglink_R

Annealing Temperature:  60C

Run using samples after PCR purification.

PCR of Syk-(15aa) (5% DMSO)

PCR of TEVp

Gel shows successful PCR of TEVp from pENTR_L1_Tev2.

Forward primer:  iGEM_AS010 TEVp Qlonglink_F

Reverse primer:  iGEM_AS011 TEVp QX_R

Annealing Temperature:  60C

Run using samples after PCR purification.

Restriction Digest Verification of pENTR

1 July 2014:

See gel here