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Cloak
id7-2
Golden Gate Variable Expression Vector: Low Sensor (6-27)

Mini-preped EF colonies.
40/50 ng/µL.

PCR NcoI-mRFP-NsiIDigest NcoI-mRFP-NsiILigation
Primers arrived.
PCR products PCR-ed some more.		mRFP-PCR product digested concurrently with NcoI and NsiI.
Digestion begun at 1922.		mRFP-PCR-product digestion is ligated with digested MAV-1212.
MAV1212-RFP Propagation (7-2)
MAV1212-mRFP ligation products transformed into cells.
Plated and left in the incubator at 0043 of next day.

 

 

14-7-3

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id7-3

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id7-3
MAV1212-RFP Propagation (7-2)
Plated some more cells.
Left in incubator at 2015

 

 

 

14-7-7

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id7-7

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id7-7
 MAV1212-RFP Propagation (7-2)

Red (actually barely pinkish) colonies, hallelujah.
Colonies picked for shaker at 2010