14-8-7
| pENTR-L1-TALER14-L2 Digest AseI 3.1 |
|---|
|
14-8-8
| LR Gateway |
|---|
MAV1212-RFP
hEF1a
TAL14 MAV1212-RFP
hEF1a
TAL21 |
14-8-11
| Transformation |
|---|
MAV1212-hEF1a-TALER14 MAV1212-hEF1a-TALER21 |
14-8-15
I dont understand i dont know i dont
14-8-18
| LR |
|---|
MAV1212-mRFP
pENTR-L4-hEF1a-R1
pENTR-L1-TALER14-L2 |
14-8-19
| Transformation |
|---|
| MAV1212-hEF1a-TALER14 |
14-8-20
| Propagation | LR |
|---|
| MAV1212-hEF1a-TALER14 | MAV1212-mRFP pENTR-L4-hEF1a-R1 pENTR-L1-TALER14-L2 |
14-8-21
| Digest | Transformation |
|---|
| MAV1212-hEF1a-TALER14: | MAV1212-hEF1a-TALER14 |
| DNA | Con (ng/µL) | Enzyme | Buffer | Vol DNA | Mass DNA | Vol Enzyme | Vol Buffer | Vol Water | Vol Total |
| Ladder | | | | | | | | | |
| TAL14 Insert 1 | 282.5 | PstI | 3.1 | 3.54 | 1000 | 1 | 2 | 13.46 | 20 |
| TAL14 Insert 2 | 316.9 | PstI | 3.1 | 3.16 | 1000 | 1 | 2 | 13.84 | 20 |
| TAL14 Control | 316.9 | | | 3.16 | 1000 | | | 16.84 | 20 |
