Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Alternative backbones? Check iGEM 2013
- Actual list of experiments
- Find primers for sequencing
miRNA Sensors (Lab Work)
- Sequence/digest plasmids to verify
- EF
- GH
- pEXPR: MAV1212-Hef11a-eBFP2
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- Pick colonies and grow up
- MAV1212-mRFP
- L1_TALER14_L2
- pTAL14-mKate-pEST
- pENTR: TAL14 (promoter)
- Miniprep
- GH
- Transformations
- GH (Golden Gate)
- MAV1212-Hef1a-L7ae (LR)
- MAV1212-Hef1a-TAL14 (LR)
NOTES