Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Can we quantify repression?
miRNA Sensors (Lab Work)
- Pick pENTR TAL14
- Digest and Run gel
- MAV1212-hEF1a-TAL21
- Flow Cytometry
- TAL14 tuning experiment
- Pick colonies
- miR-125b (TAL14)
- Transform
- MAV1212-hEF1a-TAL21
- Double input low sensor
NOTES