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People to ContactEmailQuestionsContacted Yet?

Jacob Beal

jakebeal@bbn.com

Questions

Had first meeting

Jeremy Gam

jgam@mit.edu

QuestionsIn close contact
Jin Huh

jhuh@mit.edu

QuestionsHad first meeting

 

Long Term Goals

Building the Cell Detector Circuit

  •  Choose profile
  •  Verify sensors
  •  Create mathematical model

Experiments

    •  L7ae repression
    •  TAL14 repression
    •  TAL21 repression
    •  Single input sensors
    •  Multiple input sensors

Alzheimer's Model

  •  Agree on a team wide, full Alzheimer's model

Today's Agenda

How To Please Ron/Brain

miRNA Sensors (Theory)

  •  FIND pENTR: TAL21 
  •  TASBE tools with Brian
  •  Anaylze sequencing results
  •  Pick up sensors from Jeremy
  •  Clean up experiments page

miRNA Sensors (Lab Work)

  •  Transform
    •  hEF1a-TAL14 (LR)
    •  Sensors from Jeremy? 
  •  Inoculate midi culture
    •  Double input low sensor
  •  Tissue Culture
    •  Seed plate for miRNA sensors w/o siRNA
  •  Check dilutions in TC room

NOTES