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Progress
| Cloning | Transfection | Dox | Cytometry | Data Analyis |
|---|---|---|---|---|
| LRs transformed |
Procedure
Background
To determine the optimal level of expression of BCR components and Syk-TEVp, we are placing them under the control of inducible/repressible promoters. This experiment aims to characterize the activity of the promoters we are going to use: TREt and pLac.
Approach
We are going to investigate the expression of mKate driven by each of the promoters with varying concentrations of inducer (dox) and repressor (IPTG).
Parts
| Part | Status |
|---|---|
| hEF1a:rtTA | |
| TRE:mKate | |
| pLac:mKate | |
| hEF1a:LacI |