...
*performed a T-test on data (albeit sketchy T-test, given that there were only 2 duplicates of each condition) --> suspended vs. adherent show no significant difference in co transfection efficiency |
Discussion:
Bleed-through:
We should run single color controls to account for bleed-through. The gating that gave the above data excluded a population when gating in the yellow vs blue space on account of bleed through. However, we can't know for sure that that population is just bleed through without single color controls.
Non-linear plot:
y is just y=t
x=0.1t+2
So early on the +2 dominates, but at the higher domain the 0.1t dominates. That is why the plot looks like it does. All a*x vs b*y linear functions are straight diagonal lines. ax+c looks like ax for ax>>c, and like c for ax<<c. and a constant makes a vertical lines on log log axis. That is why it starts pointing up then tilts over and makes a straight diagonal.
