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Propagating MAV1212 and MAV1212-Hef1a-EBFP2

Stock Concentrations:

EBFP2: 1118 ng/µL

MAV: unknown.

1856: Diluted DNA samples for both x100 before transformation

4 Plates, LO HI for both.

2144: Culture plates incubated overnight at 30°C at for 16-18 hours.

 

 

14-6-18

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Propagating MAV1212 and MAV1212-Hef1a-EBFP2
1426: Checked cell plates. EBFP2 produced colonies. MAV1212 overgrown.
MAV1212 StreakingEBFP2 Pick Colonies
2044: Picked single colonies from each plate and streaked over xgal plates.
Let on the incubator overnight at 30°C for 16-18 hours.		2040: Picked one colony each from the HI and LO plates and immersed in LB Media.
Left on the shaker incubator overnight.

 

 

6-19-2014

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MAV1212 Streaking
1254: Disaster. No colonies.