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Cloak

id	6-19

MAV1212 Streaking
1254: Disaster. No colonies.

Propagating MAV1212 and MAV1212-Hef1a-EBFP2 (Again)

Concentrations:

EBFP2: 12 ng/µL

MAV: 4 ng/µL

4 Plates, LO HI for both.

1715: Culture plates inseerted into 30°C incubator.

14-6-20

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id	6-20

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id	6-20

Propagating MAV1212 and MAV1212-Hef1a-EBFP2 (Again)
1105: Low transformation rate. Suspicions fall on faulty plates. Plating to be redone later with existing samples and streaking.

14-6-23

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id	6-23

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id	6-23

Colony Picking MAV1212

Propagation MAV1212

Attempted to isolate monoclonal colony from MAV1212 lawn.

Inserted into Amp+ LB medium.

Left on incubator shaker at 2114.

Re-plated transformation suspension on Amp+ plates.

Streaked colonies from lawn.

Left on 30°C incubator at 2120.

14-6-24

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id	6-24

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id	6-24

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