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NAMEDependency      
GROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR hEF1a: Gmab Heavy

pENTR Gmab Heavy

pENTR hEF1a

Antibodiesdesignedexpression      
pEXPR hEF1a: Gmab Light

pENTR Gmab Light

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79A

pENTR CD79A+stop

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79B

pENTR CD79B+stop

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79A-TCS-Gal4VP16

pENTR CD79A-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16

pENTR CD79B-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: Lyn

pENTR Lyn+stop

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR TRE: Lyn-mKate

pENTR Lyn-mKate

pENTR PTet

Antibodiesdesigned expression      
pEXPR TRE: Syk-eYFP

pENTR Syk-eYFP

pENTR PTet

Antibodiesdesigned expression      
pEXPR PTet: Syk-TEVp

pENTR Syk-TEVp

pENTR PTet

Antibodiesdesigned expression      
pEXPR TRE: eBFPpENTR eBFPAntibodiesin storage?expression      
pEXPR Gal4/UAS: mKate-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTApENTR rtTAAntibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Tre-TEVp-Antibodiesin labexpression      
pENTR Gmab HeavygBlock mIgM Gmab HeavyAntibodiesOrderedentry   IDT  
pENTR Gmab LightgBlock mIgM Gmab LightAntibodiesOrderedentry   IDT  
pENTR CD79A (pDONR223)-Antibodiesin storageentryiGEM -20  DNASU  
pENTR CD79A+stoppENTR CD79A (pDONR223)Antibodiesin constructionentry      
pENTR CD79B (pDONR223)-Antibodiesin storageentryiGEM -20  DNASU  
pENTR CD79B+stoppENTR CD79B (pDONR223)Antibodiesin constructionentry      
pENTR CD79A-TCS-Gal4VP16pENTR CD79A (pDONR223)
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4		Antibodiesin constructionentry      
pENTR CD79B-TCS-Gal4VP16

pENTR CD79B
pENTR Gal4VP16

Antibodiesin constructionentry      
pENTR Lyn (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Lyn+stoppENTR Lyn (pDONR223)Antibodiesin constructionentry      
pENTR Lyn-mKatepENTR Lyn
pENTR mKate		Antibodiesin constructionentry      
pENTR Syk (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Syk+stoppENTR Syk (pDONR223)Antibodiesin constructionentry      
pENTR Syk-eYFPpENTR Syk
pENTR eYFP		Antibodiesin constructionentry      
pENTR Syk-TEVppENTR Syk
pEXPR Tre-TEVp		Antibodiesin constructionentry      
pENTR eBFP-Antibodiesin storage?entry      
pENTR L1_mKate_L2-Antibodiesin storageentry      
pENTR rtTA-Antibodiesin storageentry      
pENTR_L1_EYFP_L2-Antibodiesin storageentry      
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4-Antibodiesin storage

entry

iGEM -20KBN/AVeliaHigh 
gBlock mIgM Gmab Heavy-AntibodiesorderedgBlock   IDT  
gBlock mIgM Gmab Light-AntibodiesorderedgBlock   IDT  
pEXPR Tre: BACE2-YFP
  • pENTR L1--BACE2-eYFP–L2
    • Q1-BACE2-Q2
      • BACE2
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentin constructionexpression      

pENTR L1--BACE2-eYFP--L2

  • Q1-BACE2-Q2
  • Q2-eYFP-QX
Treatmentin constructionentry      

Q1-BACE2-Q2

BACE2Treatmentin constructionPCR product      
BACE2n/aTreatmentin the labexpression      

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR product      
pEXPR eYFPn/aTreatmentin the labexpression      

pENTR Tre

n/aTreatmentin the labentry      

pDEST R4--R2

n/aTreatmentin the labdestination      
pEXPR Tre: BACE1-YFP
  • pENTR L1--BACE1-eYFP–L2
    • Q1-BACE1-Q2
      • BACE1
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentplanned expression      

pENTR L1--BACE1-eYFP--L2

  • Q1-BACE1-Q2
  • Q2-eYFP-QX
Treatmentplannedentry      

Q1-BACE1-Q2

BACE1TreatmentplannedPCR product      
BACE1n/aTreatmentin the labexpression      

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR product      

pENTR Tre

n/aTreatmentin the labentry      

pDEST R4--R2

n/aTreatmentin the labdestination      
pEXPR U6:mKate-miRNA(intronic) Treatmentin planningexpression      
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic) Treatmentin planningexpression      

ggDONR L1_LacZ_L2

 Receptorin storageplasmid+4 in iGEM roomLA- Kyle 
Expand
title...

not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough

ggDONR L1_LacZ_L2 (New)

 Receptorin storageplasmid+4 in iGEM roomLA-Brandon 
Expand
title...

will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock

pENTR L4_Hef1a_R1

 Receptorin labplasmid     
Expand
title...

will be used to make LilrB2 expression vector

pDEST

 Receptorin labplasmid     
Expand
title...

will be used to make LilrB2 expression vector 

pEXPR Hef1a:LilrB2

pENTR L1_LilrB2_L2

pENTR L4_Hef1a_R1

Receptorin constructionwaiting on partplasmid     waiting on entry vector

pENTR L1-LilrB2-L2

 LilrB2 gBlockReceptorto be verifiedplasmid   -4 in iGEM roomSS-  
Expand
title...

 failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

LilrB2 gBlock

 Receptorin storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate

pEXPR Hef1a:PirB

pENTR L1_PirB

_L2

pENTR L4_Hef1a_R1

 

Receptorin constructionWaiting on partexpression      waiting on entry vector

pENTR L1

-

_PirB

-

_L2

PirB gBlock C terminus

PirB gBlock N terminus

 

Receptorto be verified entry     
Expand
title...

 failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

PirB gBlock C terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate

PirB gBlock N terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
PDONR MAV1212MAV1212miRNA sensingin storagedonor-20 in iGEM roomRRN/AJeremy GamHighOriginal Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2-miRNA sensingin lab?entry?RRN/AN/AHighStock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:l7aeMAV1212miRNA sensingin constructionexpressionN/ARRN/AN/AHigh 
pENTR R1_mKate_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
MAV1212 hEF1A:eBFP2MAV1212miRNA sensingin storagepositive control plasmid-20 in iGEM roomRRN/AJeremy GamMedium 
PENTR R1_eYFP_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:mKate2MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:144MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:mKate2:144:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30dMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:let7fMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:125bMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30d:let7f:125b:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
Q1_144_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_181c_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_30d_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_let7f_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_125b_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_146a_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
           

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