PCR of Syk+stop
Gel shows successful PCR of Syk+stop from pENTR Syk (pDONR223).
Forward primer: iGEM_AS004 Syk Q1_F
Reverse primer: iGEM_AS005 Syk QX_stop_R
Annealing Temperature: 55C
Run using samples after PCR purification.
Restriction Digest Verification of pENTR
1 July 2014:
See gel here
Correct plasmids | Failed plasmids |
|---|---|
Syk+stop (2) | Syk+stop (1) Syk+stop (3) Syk+stop (4) Syk+stop (5) |