...
- Highlight the gene that you want to amplify
- Select "Primers" from the top banner and "design new primers" from the drop down tab
- Make sure you have checked "Forward Primer" and "Reverse Primer"
- Set task to "precise" if you are doing this for a cloning reaction
- check "Target Region"
- Modify characteristics as needed - see "checking Tm and other primer design tips" in the protocols page
- Hit OK and pick the best primer generated - hovering your mouse over the primer will give you Tm and GC content
...