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pENTR mKatehEF1a:CD79B-TCS-G4hEF1a:Gal4VP16
MluI

BsrGI

Cuts in Gal4

BsrGI

Cuts in Gal4

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pENTR mKate, hEF1a:CD79B-TCS-Gal4VP16, hEF1a:Gal4VP16, hEF1a:CD79B-TCS-Gal4VP16 (Redone because lane 3, the original, disappeared and we were concerned we would not see bands)