| pENTR mKate | hEF1a:CD79B-TCS-G4 | hEF1a:Gal4VP16 |
| MluI | BsrGI Cuts in Gal4 | BsrGI Cuts in Gal4 |
 |  |  |
pENTR mKate, hEF1a:CD79B-TCS-Gal4VP16, hEF1a:Gal4VP16, hEF1a:CD79B-TCS-Gal4VP16 (Redone because lane 3, the original, disappeared and we were concerned we would not see bands)