...
- Transform all LRs (hEF1a:PT, hEF1a:LT, hEF1a:TC, TREt:TC)
- Pick hEF1a:YC colonies
- Miniprep TREt:YC if it worked. Otherwise, wait for Brian. Worked.
- Test LilrB2TCSG4 digest in random places. Is the main LilrB2 stock missing a chunk? Preposterous. It's fine.
- PCR C term YFP? Nope, brian's looking into it.
- Midiprep inoculate TREt:YC
...
Ran PT (pENTR_PirBTCSG4) to make sure it isn't contaminated).
The gel for PT had an extra band, but the sequencing results show that the TCSG4 is present and goes into the PirB region. That means everything is in order 
