All Times in Zulu
14-6-17
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| id | 6-17 |
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| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 |
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Stock Concentrations: EBFP2: 1118 ng/µL MAV: unknown. 1856: Diluted DNA samples for both x100 before transformation 4 Plates, LO HI for both. 2144: Culture plates incubated overnight at 30°C for 16-18 hours. |
14-6-18
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| id | 6-18 |
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| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 |
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| 1426: Checked cell plates. EBFP2 produced colonies. MAV1212 overgrown. |
| MAV1212 Streaking | EBFP2 Pick Colonies |
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| 2044: Picked single colonies from each plate and streaked over xgal plates. Let on the incubator overnight at 30°C for 16-18 hours. | 2040: Picked one colony each from the HI and LO plates and immersed in LB Media. Left on the shaker incubator overnight. |
6-19-2014
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