Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build Choose profile
- Verify sensors
- Create mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
Lab contacts
- Email our contacts
- Finalize list of questions for our contacts
miRNA
- Finish miRNA profile explanations
- Pathways it's involved in
- How up/down regulated is it? (T Test)
- List of cells we have access to
- Email Samira about what she just ordered
- Break down sensor assembly plan (from Brian)
- Start drafting preliminary experiments
Presentation
- Divide labor for next week's presentation slides
What to do, what to do...
Expression levels of each miRNA in profile in each cell lineNowhere to be found on the internet. If we want to know it, we're going to have to do the assays ourselves (as a mini experiment)How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Re-analyze all of our data in MATLAB (Gary)
- Learn how to acquire MATLAB from Brian
- Find natural concentrations of relevant miRNAs.
miRNA Sensors (Lab Work)
- Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
- Mini-prep MAV1212 hEF1A Tal14
- Digest and Map MAV1212 hEF1A Tal14
- Transform third LR trial if not verified
- Pick/Streak hEF1a: Gal4VP16 2A rtTA3
NOTES:
hEF1a: Gal4VP16 2A rtTA3 culture did not grow so