| People to Contact | Questions |
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| Contacted Yet? |
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Jacob Beal |
Fusun Yaman
Fusun@bbn.com
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Toward Automated Design of Cell Detectors
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Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells
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Ways to profile miRNA
- Experimentally
- Computer Models
- Choose profile
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Modeling the Cell Detector Circuit
- Proto Simulation
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Building the Cell Detector Circuit
- Understand circuit diagrams
- Design mini experiments
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Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Re-analyze all of our data in MATLAB (Gary)
- Learn how to acquire MATLAB from Brian
- Find natural concentrations of relevant miRNAs.
miRNA Sensors (Lab Work)
- Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
- Mini-prep MAV1212 hEF1A Tal14
- Digest and Map MAV1212 hEF1A Tal14
- Transform third LR trial if not verified
- Pick/Streak hEF1a: Gal4VP16 2A rtTA3
NOTES:
hEF1a: Gal4VP16 2A rtTA3 culture did not grow so