Page History

Long Term Goals

•  Read literature
•  Choose profile

Building the Cell Detector Circuit

•  Build Choose profile
•  Verify sensors
•  Create mathematical model
•  Make test bed
  •  Controlled miRNA expression
  •  Constructed miRNA sensors

Mini experiments

• •  Single miRNA detector circuit

Experiments

• •  L7ae repression
  •  TAL14 repression
  •  TAL21 repression
  •  Single input sensors
  •  Multiple input sensors

Alzheimer's Model

•  Agree on a team wide, full Alzheimer's model
  

Today's Agenda

Presentations

How To Please Ron/Brain

•  Keep updating the parts tree (include experiments Run through NEGEM Presentation (12:00)

miRNA Sensors (Theory)

•  Create circuit diagram (Raymond)
  •  Put it on our subgroup home page
•  Determine final output of circuit
•  How does repressor affect the circuit?
  
  •  Determine our repressors and repressible promoters 
  •  Find entry vectors in our library
•  Understand why we're doing any of these reactions
• Re-analyze all of our data in MATLAB (Gary)
•  Learn how to acquire MATLAB from Brian
•  Find natural concentrations of relevant miRNAs.

miRNA Sensors (Lab Work)

•  Transform MAV1212 into ccdB resistance cells
  •  Grow at low temp w/ x-gal, shielded from light
  •  Pick as many colonies as possible
  •  Sequence ccdB region
•  Propogate plasmids (in room 215, in -20 fridge, labeled "igem 2014")
  •  MAV1212
  •  MAV1212-Hef1a-EBFP2
  •  (MAV1212-Hef1a-mKate2)
 Gateway MAV1212-Hef1a-L7ae

Cell Models

•  Finish table of concentrations in each cell line
•  Finalize siRNA transfection technique 
  

Social Media

•  Public wiki
  •  Paragraph from sponsorship booklet

• Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
•  Mini-prep MAV1212 hEF1A Tal14
•  Digest and Map MAV1212 hEF1A Tal14
•  Transform third LR trial if not verified
•  Pick/Streak hEF1a: Gal4VP16 2A rtTA3

NOTES:

hEF1a: Gal4VP16 2A rtTA3 culture did not grow so