Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build Choose profile
- Verify sensors
- Create mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
Ask Jeremy
- Why genome integration with bxb1 and puro?
- Will this work with transient transfection?
Presentations
- Double check what NEGEM looks like
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Re-analyze all of our data in MATLAB (Gary)
- Learn about Jacob Beal's work and how it applies to us
- Make diagram to connect circuit diagram to plasmid construction map
- to acquire MATLAB from Brian
- Find natural concentrations of relevant miRNAs.
miRNA Sensors (Lab Work)
- Check MAV1212 and MAV1212-Hef1a-EBFP2 plates
- Pick as many colonies as possible to grow overnight
- Miniprep them
- Sequence ccdB region
- Transform MAV1212-Hef1a-mKate2 (if ready)
- Gateway MAV1212-Hef1a-L7ae
- Find Hef1a
- Find L7ae
Cell Models
- Finish table of concentrations in each cell line
- Finalize siRNA transfection technique
Social Media
- Check if Alexa handled public wiki
- Add more to facebook page
- Keep Twitter live
- Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
- Mini-prep MAV1212 hEF1A Tal14
- Digest and Map MAV1212 hEF1A Tal14
- Transform third LR trial if not verified
- Pick/Streak hEF1a: Gal4VP16 2A rtTA3
NOTES:
hEF1a: Gal4VP16 2A rtTA3 culture did not grow so