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titleName Conventions

For priority:  1 = top priority (absolutely necessary), 2 = middle priority (would be useful), 3 = low priority (only if we have time)

 

...

pENTR L1-Gene name-L2

pEXPR Promoter:Gene

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titleStatus Words
WordMeaning
in planningbeing designed in geneious
designed

in planning 

plasmid design completed in geneious
in labsomeone else in the lab has it

...

ordered 
in

...

construction 
waiting on partdependent on part that is in construction
to be verified 
in storage

we have it in our fridge/freezer, etc

NAMEDependency      

constructed 

in storage 

 

plasmid
NAMEGROUPSTATUSTYPELOCATIONINITIALSCONSTRUCTED ONOBTAINED FROMPRIORITYNOTES
pEXPR Gal4UAS:eYFPAntibodyhEF1a: Gmab Heavy

pENTR Gmab Heavy

pENTR hEF1a

Antibodiesdesignedin someone else's freezerexpression      
pEXPR Gal4UAS:mKateAntibodyhEF1a: Gmab Light

pENTR Gmab Light

pENTR hEF1a

Antibodiesdesigned in someone else's freezerexpression      
pEXPR Tre-TEVpAntibodyhEF1a: CD79A

pENTR CD79A+stop

pENTR hEF1a

Antibodiesdesigned in someone else's freezerexpression      Hef1a-rtTaAntibody
pEXPR hEF1a: CD79B+stop

pENTR CD79B+stop

pENTR hEF1a

Antibodiesdesigned in someone else's freezerexpression      
pEXPR hEF1a: CD79A-TCS-Gal4VP16

pENTR CD79A-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression      
pEXPR hEF1a: CD79B-TCS-Gal4VP16

pENTR CD79B-TCS-Gal4VP16

pENTR hEF1a

Antibodiesdesigned expression   CD79A WTAntibodypossibly mislabled DNA and frozen stock DNA in freezer, frozen bacteria stock in -80   
pEXPR hEF1a: Lyn+stop

pENTR Lyn+stop

pENTR hEF1a

Antibodiesdesigned expression  CD79B WT    
pEXPR TRE: Lyn-mKate

pENTR Lyn-mKate

pENTR PTet

Antibodiesdesigned expression Antibodyminiprepped, awaiting sequence verification DNA in freezer, frozen bacteria stock in -80     
pEXPR TRE: Syk-eYFP

pENTR Syk-eYFP

pENTR PTet

Antibodiesdesigned expressionCD79A-TCS-Gal4VP16Antibodyplanned       CD79B-TCS-Gal4VP16Antibodyplanned 
pEXPR PTet: Syk-TEVp

pENTR Syk-TEVp

pENTR PTet

Antibodiesdesigned expression      
pEXPR TRE: eBFPpENTR eBFPAntibodiesin storage?expression      
pEXPR Gal4/UAS: mKate-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR hEF1a: rtTApENTR rtTAAntibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Gal4UAS:eYFP-Antibodiesin storageexpressioniGEM -20CRN/AVeliaHigh 
pEXPR Tre-TEVp-Antibodiesin labexpression Lyn WTAntibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80     
pENTR Gmab HeavygBlock mIgM Gmab HeavyAntibodiesOrderedentryLyn-eYFPAntibodyplanned   IDT  
pENTR Gmab LightgBlock mIgM Gmab LightAntibodiesOrderedentry   IDT Lyn point mutated (always phosphorylate) 
pENTR CD79A (pDONR223)-Antibodiesin storageentryiGEM -20  DNASUAntibodyresearching  
pENTR CD79A+stoppENTR CD79A (pDONR223)Antibodiesin constructionentry      
pENTR CD79B (pDONR223)-Antibodiesin storageentryiGEM -20

Syk WT

Antibodyminiprepped, need to nanodrop and sequence DNA in freezer, frozen bacteria stock in -80  DNASU   
pENTR CD79B+stoppENTR CD79B (pDONR223)Antibodiesin constructionentrySyk-eYFP (mutant non phosphorylate)Antibodyplanned       Syk-TEVpAntibody
pENTR CD79A-TCS-Gal4VP16pENTR CD79A (pDONR223)
pEXPR hEF1a-FGFR-mKate-TCS(L)-G4		Antibodiesin constructionentryplanned      
pENTR CD79B-TCS-Gal4VP16

pENTR CD79B
pENTR Gal4VP16

Antibodiesin constructionentry GMab kAntibodyon order      
pENTR Lyn (pDONR223)-Antibodiesin storageentryiGEM -20 GMab H Antibodyaddgeneon order  
pENTR Lyn+stoppENTR Lyn (pDONR223)Antibodiesin constructionentry      
pENTR Lyn-mKatepENTR Lyn
pENTR mKate		Antibodiesin constructionentryGMabH-TCS-Gal4VP16Antibodyplanned       
pENTR Syk (pDONR223)-Antibodiesin storageentryiGEM -20  addgene  
pENTR Syk+stoppENTR Syk (pDONR223)Antibodiesin constructionentryhsa-miR-181cmiRNAplanned       hsa-miR-9miRNAplanned
pENTR Syk-eYFPpENTR Syk
pENTR eYFP		Antibodiesin constructionentry       hsa-miR-30d-5p
pENTR Syk-TEVppENTR Syk
pEXPR Tre-TEVp		Antibodiesin constructionentrymiRNAplanned       
pENTR eBFPhsa-miR-144-5pmiRNA-Antibodiesin storage?entryplanned       hsa-let-7f-5pmiRNA
pENTR L1_mKate_L2-Antibodiesin storageentryplanned       
pENTR rtTAhas-miR-125bmiRNAAntibodiesin storageentryplanned       has-miR-146amiRNAplanned
pENTR_L1_EYFP_L2-Antibodiesin storageentry       
pEXPR TRE-t:BACE2-eYFPhEF1a-FGFR-mKate-TCS(L)-G4-Antibodiesin storage

entry

iGEM -20KBN/AVeliaHigh 
gBlock mIgM Gmab Heavy-AntibodiesorderedgBlock   IDT  
gBlock mIgM Gmab Light-AntibodiesorderedgBlockTreatmentplanned     IDT  
pEXPR Tre: BACE2-YFP
  • pENTR L1--BACE2-eYFP–L2
    • Q1-BACE2-Q2
      • BACE2
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
    • pENTR L4--TRE-t--R1
Treatmentin the lab alreadyconstruction expression      

pENTR L1--BACE2-eYFP--L2

  • Q1-BACE2-Q2
  • Q2-eYFP-QX
Treatmentplannedin constructionentry      

Q1-BACE2-Q2

BACE2Treatmentin constructionPCR product       
BACE2 (HsCD00076140)n/aTreatmentin -80the labexpression      

Q2-eYFP-QX

pEXPR eYFPTreatmentin the labPCR productplasmid AL     
pEXPR eYFPn/aTreatmentin the lab alreadyexpression      

pENTR Tre

n/aTreatmentin the labentry      

pDEST R4--R2

n/aTreatmentin the lab already destination      
pEXPR Hef1a:BACE1-eYFPTre: BACE1-YFP
  • pENTR L1--BACE1-eYFP–L2
    • Q1-BACE1-Q2
      • BACE1
    • Q2-eYFP-QX
      • pEXPR eYFP
  • pENTR Tre
  • pDEST R4--R2
Treatmentplanned  expression      

pENTR

L4--Hef1a--R1		Treatmentin the lab already

L1--BACE1-eYFP--L2

  • Q1-BACE1-Q2
  • Q2-eYFP-QX
Treatmentplannedentry       
pENTR L1

Q1-BACE1-Q2

BACE1-eYFP--L2Treatmentplanned PCR product      
BACE1n/aTreatmentin the labexpressionto be ordered       

Q2-eYFP-QX

pEXPR eYFPTreatmentin the lab already PCR product      

pENTR Tre

n/aTreatmentin the labentry      

pDEST R4--R2

n/aTreatmentin the lab already destination      
pEXPR U6:mKate-miRNA(intronic) Treatmentplannedin planning expression      
pEXPR U6/TRE-t(hybrid promoter):mKate-miRNA(intronic)Treatmentplanned  Treatment in planning expression   Experiment 1    

ggDONR L1_LacZ_L2

     
  • ggDONR
Receptorin storageplasmid+4 in iGEM roomLA- Kyle not sure if blue/white selection is working or not; conducted troubleshooting experiment - problem was with plates; however not sure if concentration is high enough

 

 

ggDONR L1_LacZ_L2 (New)

 
  • ggDONR New
Receptorin storageplasmid+4 in iGEM roomLA-Brandon 
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title...

will potentially use if original ggDONR is not working; tested - gives a lot more blue colonies than original ggDONR; will pick colony to make a cell stock

pENTR L4_Hef1a_R1

 Hef1a Entry VectorReceptorin labplasmid     
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title...

will be used to make LilrB2 expression vector

pDEST

Destination Vector Receptorin labplasmid     
Expand
title...

will be used to make LilrB2 expression vector

 

pEXPR Hef1a:LilrB2

pENTR L1_LilrB2_L2

pENTR L4_Hef1a_R1

LirB2 Expression Vector

Receptorin constructionwaiting on partplasmid     waiting on entry vector

pENTR L1-LilrB2-L2

LilrB2 Entry VectorgBlockReceptorin constructionto be verified -4 in iGEM roomSS-  
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title
 ifailing
...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

LilrB2 gBlock

 Receptorin storagegBlock-20 in iGEM roomLA- IDT used in Golden Gate

pEXPR Hef1a:PirB

pENTR L1_PirB_L2

pENTR L4_Hef1a_R1

PirB Expression Vector

Receptorin constructionWaiting on partexpression      waiting on entry vector

pENTR L1_PirB_L2

PirB gBlock C terminus

PirB

Entry Vector

gBlock N terminus

Receptorto be verifiedentryin construction     
Expand
title...

 

failing to get liquid cultures to grow in order to miniprep to extract plasmid DNA

PirB gBlock C terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate

PirB gBlock N terminus

 Receptorin storagegBlock-20 in iGEM roomLA-IDT used in Golden Gate
PDONR MAV1212MAV1212miRNA sensingin storagedonor-20 in iGEM roomRRN/AJeremy GamHighOriginal Multi Assembly Vector, currently under cloning
pENTR L1_hEF1A_L2-miRNA sensingin lab?entry?RRN/AN/AHighStock in iGEM room may exist, but the one I found was empty
pENTR R1_l7ae_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:l7aeMAV1212miRNA sensingin constructionexpressionN/ARRN/AN/AHigh 
pENTR R1_mKate_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
MAV1212 hEF1A:eBFP2MAV1212miRNA sensingin storagepositive control plasmid-20 in iGEM roomRRN/AJeremy GamMedium 
PENTR R1_eYFP_L4-miRNA sensingin storageentry-20 in iGEM roomRRN/AN/AHigh 
pEXPR hEF1A:mKate2MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:144MAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:mKate2:144:181cMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30dMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:let7fMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:125bMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
pEXPR hEF1A:eYFP:30d:let7f:125b:146aMAV1212

miRNA sensing

in planningexpressionN/ARRN/AN/AHigh 
Q1_144_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_181c_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_30d_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_let7f_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_125b_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh 
Q1_146a_Q2miRNA target sitemiRNA sensingin labtarget siteJeremy Gam's LabRRN/AIDTHigh