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Comment: Task marked complete

 

 

People to ContactEmailQuestionsContacted Yet?

Jacob Beal

jakebeal@bbn.com

Questions

Had first meeting

Jeremy Gam

jgam@mit.edu

QuestionsIn close contact
Jin Huh

jhuh@mit.edu

QuestionsHad first meeting

 

Long Term Goals

miRNA Profile

  •  Read literature
  •  Choose profile

Building the Cell Detector Circuit

  •  Build  Choose profile
  •  Verify sensors
  •  Create mathematical model
  •  Make test bed
    •  Controlled miRNA expression
    •  Constructed miRNA sensors

Mini experiments

    •  Single miRNA detector circuit

Experiments

    •  L7ae repression
    •  TAL14 repression
    •  TAL21 repression
    •  Single input sensors
    •  Multiple input sensors

Alzheimer's Model

  •  Agree on a team wide, full Alzheimer's model

Today's Agenda

How To Please Ron/Brain

miRNA Sensors (Theory)

  •  Plan out chaining multiple miRNA target sites together
    •  Meet with Jeremy?
    Re-analyze all of our data in MATLAB (Gary)
  •  Learn how to acquire MATLAB from Brian
  •  Find natural concentrations of relevant miRNAs.

miRNA Sensors (Lab Work)

  •  TranformLRs 
    •  MAV1212-Hef1a-mKate2
    •  MAV1212-Hef1a-L7ae
  •  Pick colonies from transformations
    •  MAV1212
    •  MAV1212-Hef1a-eBFP2
    •  Hef1a
  • Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
  •  Mini-prep MAV1212 hEF1A Tal14
  •  Digest and Map MAV1212 hEF1A Tal14
  •  Transform third LR trial if not verified
  •  Pick/Streak hEF1a: Gal4VP16 2A rtTA3

NOTES:

hEF1a: Gal4VP16 2A rtTA3 culture did not grow so