Long Term Goals
Ways to profile miRNA
- Experimentally
- Computer Models
- Choose profile
Modeling Building the Cell Detector Circuit
- BioCompiler/Proto/MATLAB Simulation
Building the Cell Detector Circuit
- Understand circuit diagrams
Mini experiments
- Design single miRNA detector circuit
- Create up/downregulated miRNA models
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Short Term Goals
miRNA
- Explain why we chose the miRNA we chose
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Re-analyze all of our data in MATLAB (Gary)
- Learn how to acquire MATLAB from Brian
- Find natural concentrations of relevant miRNAs.
miRNA Sensors (Lab Work)
- Midi-prep hEF1a:rtTA and hEF1a: Gal4VP16 2A rtTA3
- Mini-prep MAV1212 hEF1A Tal14
- Digest and Map MAV1212 hEF1A Tal14
- Transform third LR trial if not verified
- Pick/Streak hEF1a: Gal4VP16 2A rtTA3
NOTES:
hEF1a: Gal4VP16 2A rtTA3 culture did not grow so