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Inducible and Constitutive Cascades
Syk-TEVp Cleavage
| Number | Name | Parts/ReagentsStatus |
|---|
| 1 | Membrane Localization of BCR | | Expand |
|---|
| - B Cells
- hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- Cover Slips
- 0.1% Gelatin
- RPMI1640
- Sodium Azide
- Fixation Buffer
- Parafilm
- Triton X-100
- Embedding Solution
- Anti IgM Green
|
| Ongoing |
| 2 | Beta Amyloid Binding of Gmab in BCR Complex | | Expand |
|---|
| - hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- Cover Slips
- 0.1% Gelatin
- Sodium Azide
- Fixation Buffer
- Parafilm
- Triton X-100
- Embedding Solution
- Biotinylated aBeta
- Oligomerized Biotinylated aBeta
- Streptavidin-RED
|
| Ongoing |
| 3 | Syk Western Blotexpand | Complete |
|
| |
| 4 | Membrane Localization of Lyn | Waiting on Membrane Localization |
| 54 | Membrane Localization of Lyn | | Expand |
|---|
| - hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- TRE: Lyn-mKate
- hEF1a: rtTA
- Dox
- Biotinylated aBeta
|
|
| 5 | Membrane Localization of Syk | | Expand |
|---|
| - hEF1a: Gmab L
- hEF1a: Gmab H
- hEF1a: CD79A
- hEF1a: CD79B
- hEF1a: Lyn
- TRE: Syk-eYFP
- hEF1a: rtTA
- Biotinylated aBeta
|
|
| 6 | Inactive TANGO Test | | Expand |
|---|
| - hEF1a: Gmab H
- hEF1a: Gmab L
- hEF1a: CD79A-TCS-Gal4VP16
- hEF1a: CD79B-TCS-Gal4VP16
- hEF1a: Lyn
- TRE: Lyn-TEV
- TRE: Syk-TEV
- hEF1a: rtTA
- UAS: eYFP
- Dox
|
|
7 | Active TANGO Test | | Expand |
|---|
|
- hEF1a: Gmab H
- hEF1a: Gmab L
- hEF1a: CD79A-TCS-Gal4VP16
- hEF1a: CD79B-TCS-Gal4VP16
- hEF1a: Lyn
- TRE: Lyn-TEV
- TRE: Syk-TEV
- hEF1a: rtTA
- UAS: eYFP
- Biotinylated aBeta
- Anti IgM Green
| Syk | Waiting on Membrane Localization |
| 6 | Syk-TEV Cleavage | Ready |
| 7 | Lyn-TEV Cleavage | Waiting on Parts |
| 8 | Syk-TEV & Lyn Cleavage | Waiting on Parts |
Template
| Expand |
|---|
|
| Expand |
|---|
| Well 1 HEK293 | Well 2 HEK293 | Well 3 HEK293 | Well 4 HEK293 | Well 5 HEK293 | Well 6 HEK293 | Well 7 HEK293 | Well 8 HEK293
| Well 9 HEK293 | Well 10 HEK293 | Well 11 HEK293 | Well 12 HEK293 | Well 13 HEK293 | Well 14 HEK293 | Well 15 HEK293 | Well 16 HEK293 | Well 17 HEK293 | Well 18 HEK293 | Well 19 HEK293 | Well 20 HEK293 | Well 21 HEK293 | Well 22 HEK293 | Well 23 HEK293 | Well 24 HEK293 |
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