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Zulu Time All.
14-6-17
| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 |
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Stock Concentrations: EBFP2: 1118 ng/µL MAV: unknown. 1856: Diluted DNA samples for both x100 before transformation 4 Plates, LO HI for both. 2144: Culture plates incubated overnight at 30°C for 16-18 hours. |
14-6-18
| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 |
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| 1426: Checked cell plates. EBFP2 produced colonies. MAV1212 overgrown. |
| MAV1212 Streaking | EBFP2 Pick Colonies |
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2044: Picked single colonies from each plate and streaked over xgal plates.
Let on the incubator overnight at 30°C for 16-18 hours. | 2040: Picked one colony each from the HI and LO plates and immersed in LB Media.
Left on the shaker incubator overnight. |
14-6-19
| MAV1212 Streaking |
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| 1254: Disaster. No colonies. |
| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 (Again) |
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Concentrations: EBFP2: 12 ng/µL MAV: 4 ng/µL 4 Plates, LO HI for both. 1715: Culture plates inseerted into 30°C incubator. |
14-6-20
| Propagating MAV1212 and MAV1212-Hef1a-EBFP2 (Again) |
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1105: Low transformation rate. Suspicions fall on faulty plates. Plating to be redone later with existing samples and streaking. |
14-6-23
| Colony Picking MAV1212 | Propagation MAV1212 |
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Attempted to isolate monoclonal colony from MAV1212 lawn. Inserted into Amp+ LB medium. Left on incubator shaker at 2114. | Re-plated transformation suspension on Amp+ plates. Streaked colonies from lawn. Left on 30°C incubator at 2120. |
14-6-24
| Colony Picking MAV1212 | Propagation MAV1212 |
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4. | Current MAV1212 vectors abandoned due to overactive ccdB gene killing off cells. Awaiting arrival of new vectors with better survivability. |