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Digestion Protocol
- 20 ul total
- 500-1000 ug DNA
- 1 ul enzyme
- 2 ul enzyme buffer
- fill the rest with water
- Pipette up and down thoroughly
- 37 degrees four 1-3 hours
- 4-5 ul loading dye
- gel + ladder!
An excel document is attached to aid in making calculations for multiple parallel digests easier. Use this document and attach it to your experiment for science and stuff.