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Digestion Protocol

  • 20 ul total
  • 500-1000 ug DNA
  • 1 ul enzyme
  • 2 ul enzyme buffer
  • fill the rest with water
  • Pipette up and down thoroughly 
  • 37 degrees four 1-3 hours
  • 4-5 ul loading dye
  • gel + ladder!

An excel document is attached to aid in making calculations for multiple parallel digests easier. Use this document and attach it to your experiment for science and stuff.

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