| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Update experiments page
- Obtain plasmid maps
- Email Jin about TAL14 and miRNA target sites
- Talk to Lyla/Alexa about transfecting later this week
- Look for plasmids
- pEXPR: UAS-Gal4-TAL14-eYFP
- pEXPR: Gal4VP16
- pEXPR: Hef1a-TAL14
- pEXPR: Hef1a-2x Kturn-eYFP
miRNA Sensors (Lab Work)
- Transformations
- Low Sensor Golden Gates
- pEXPR: Hef1a-eBFP2 (186 ng/uL)
- pEXPR: Hef1a-L7ae (96 ng/uL)
- Pick red colonies and grow in medium!
- Find concentrations of eBFP2 and L7ae
NOTES
Nelson doesn't know where it is, but he needs it as well, so he's on the hunt
If we're ready, we can transfect very early next week