Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Create protocol for siRNA transfection
miRNA Sensors (Lab Work)
Pick colonies
- EF
- GH
- pEXPR: Hef1a-eBFP2
- pENTR: Hefa-L7ae
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- MAV12112-Hef1a-eBFP2 (control)
NOTES