miRNA To Do List

Long Term Goals

miRNA Profile

• Read literature
• Choose profile

Building the Cell Detector Circuit

• Build mathematical model
• Make test bed
  • Controlled miRNA expression
  • Constructed miRNA sensors

Mini experiments

• • Single miRNA detector circuit

Alzheimer's Model

• Agree on a team wide, full Alzheimer's model
  

Today's Agenda

How To Please Ron/Brain

• Keep updating the parts tree (include experiments)
  • More descriptive names
  • Include other plasmid

miRNA Sensors (Theory)

• Alternative backbones? Check iGEM 2013
• Plan experiment to test Kturn (Just Kturn and L7ae)

miRNA Sensors (Lab Work)

• Pick colonies & Grow in medium
  • GH
  • pENTR: L7ae
• Midi prep
  
  • pEXPR: Hef1a-eBFP2
  • Mutant Hef1a-2xKturn-eGFP
  • Wild Type Hef1a-2xKturn-eGFP
  • EF
• Gateway
  • MAV1212-Hef1a-L7ae
• Create pENTR: TAL14
  • BP Reaction from TAL14
    • P4-donor-P1R on Brian's bench
    • TAL14-mKate pEST in Brian's fridge
• Transformation
  • BP reaction
  • TAL14 mKate

NOTES

We should sequence the low sensors when they're done