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SAMPLE
Experiment Template:
- 7/_: Seed cells
- 7/_: Transfect Promoter:Gene (___ ng/uL) and Promoter:Gene (___ ng/uL) into HEK cells
- 7/_ (_pm): Flow Cytometer
- DNA Dilution: __uL Promoter:Gene; __uL Promoter:Gene
- (Eg)x: add : (25uL Promoter:Gene-lipid complex)
| Y1 | (Y+B)1 | ////// | ////// | ////// | -ve control- dummy DNA
- with Lipofectamine
|
| Y2 | (Y+B)2 | ////// | ////// | ////// | -ve control- dummy DNA
- with Lipofectamine
|
| B1 | (Y+B)3 | ////// | ////// | ////// | -ve/-ve control |
| B2 | (Y+B)4 | ////// | ////// | ////// | -ve/-ve control |
Sample Experiment #2:
- 7/3: Seed cells
- 7/4: Transfect hEF1a:eYFP (472 ng/uL) and hEF1a:tagBFP (1176 ng/uL) into HEK cells
- 7/6 (1pm): Flow Cytometer
| Y1 | (Y+B)1 | ////// | ////// | ////// | -ve control- dummy DNA
- with Lipofectamine
|
| Y2 | (Y+B)2 | ////// | ////// | ////// | -ve control- dummy DNA
- with Lipofectamine
|
| B1 | (Y+B)3 | ////// | ////// | ////// | -ve/-ve control |
| B2 | (Y+B)4 | ////// | ////// | ////// | -ve/-ve control |
Antibody Detection
Receptor Detection
miRNA Detection
Treatment